Fakultät für Chemie - Biochemistry III
 
 
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Bielefeld University > Department of Chemistry > Research Groups > Prof. Dr. Gabriele Fischer von Mollard > Biochemistry III
EMBO J. 2003, Vol. 22, pp. 3664-3674

Use1p is a yeast SNARE protein required for retrograde traffic to the ER

Meik Dilcher, Beate Veith, Subbulakshmi Chidambaram, Enno Hartmann, Hans Dieter Schmitt, and Gabriele Fischer von Mollard

SNAREs on transport vesicles and target membranes are required for vesicle targeting and fusion. Here we describe a novel yeast protein with a typical SNARE motif but with low overall amino acid homologies to other SNAREs. The protein localized to the ER and was therefore named Use1p (unconventional SNARE in the ER). A temperature sensitive use1 mutant was generated. use1 mutant cells accumulated the ER forms of CPY and invertase. More specific assays revealed that use1 mutant cells were defective in retrograde traffic to the ER. This was supported by strong genetic interactions between USE1 and the genes encoding SNAREs in retrograde traffic to the ER. Antibodies directed against Use1p co-immunoprecipitated the SNAREs Ufe1p, myc-Sec20p and Sec22p, which form a SNARE complex required for retrograde traffic from the Golgi to the ER but neither Bos1p nor Bet1p (members of the SNARE complex in anterograde traffic to the Golgi). Therefore, we conclude that Use1p is a novel SNARE protein which functions in retrograde traffic from the Golgi to the ER.